Vector Laboratories ImmEDGE

The ImmEdge Hydrophobic Barrier Pen can be used to draw a barrier around a section on a slide that keeps aqueous solutions in place and minimizes the amount of reagent needed for each section. -Heat stable -Stable for use with buffers with and without detergent (Tween 20, Triton X-100, etc.) -Keeps reagents localized on cells and tissue preparations -Light blue barrier -Compatible with enzyme or fluorescence-based detection systems -Soluble in all commonly used clearing agents -Environmentally friendly - does not contain "ozone-depleting" substances The ImmEdge Pen is an improvement over the PAP pen for immunohistochemistry, immunocytochemistry, immunofluorescence, andin situhybridization. It is designed to provide a heat-stable, water-repellent barrier that keeps reagents localized on tissue specimens and prevents mixing of reagents when multiple sections are mounted on the same slide.TheImmEdge Pen barrier is stable for use with buffers with and without detergent (e.g. Tween 20 or Triton X-100, etc.). The pale blue barrier is insoluble in alcohol and acetone but is completely removed by all commonly used xylene and xylene substitute clearing agents. The ImmEdge Pen is compatible with enzyme or fluorescence-based detection systems. The ImmEdge Pen is environmentally friendly. It does not contain "ozone-depleting" solvents as identified by the Montreal Protocol or the Council of the European Union (specifically, fluorocarbons and chlorocarbons).